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KMID : 0613820170270091052
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Journal of Life Science
2017 Volume.27 No. 9 p.1052 ~ p.1058
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Gelidium amansii Extract, a Potent α-glucosidase and α-amylase Inhibitor, Alleviates Postprandial Hyperglycemia in Diabetic Mice
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Park Jae-Eun
Kim Jung-Min
Han Ji-Sook
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Abstract
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Gelidium amansii shows antioxidant and anti-obesity effects; however, the effect on postprandial blood glucose levels is not known. The objective of the present study was to investigate the inhibitory effect of Gelidium amansii extract (GAE) on carbohydrate-digesting enzymes and its ability to alleviate postprandial hyperglycemia in streptozotocin (STZ)-induced diabetic mice. Gelidium amansii was extracted with 80% ethanol and concentrated for use in this study. The α-glucosidase and α-amylase inhibition assays were performed using the colorimetric method. ICR normal and STZ-induced diabetic mice were orally administered GAE (300 mg/kg body weight) or acarbose (100 mg/kg body weight) alone or soluble starch (2 g/kg body weight). Blood samples were taken from the tail vein at 0, 30, 60 and 120 min. Our results indicated that GAE markedly inhibited α-glucosidase and α-amylase activities with IC50 values of 0.099±0.009 mg/ml and 0.178±0.038 mg/ml, respectively, and was a more effective inhibitor than acarbose, the positive control. Further, the postprandial blood glucose levels of STZ-induced diabetic mice in the GAE-administered group were significantly lower than those of control group mice (p<0.05). Moreover, the area under the curves (AUC) significantly decreased with GAE administration in STZ-induced diabetic mice (p<0.05). These results indicate that GAE may be effective in decreasing postprandial blood glucose levels by inhibiting carbohydrate-digesting enzymes such as α-amylase and α-glucosidase. Therefore, GAE could be used as a potential functional food for alleviating postprandial hyperglycemia.
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KEYWORD
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Gelidium amansii, postprandial hyperglycemia, α-glucosidase, α-amylase
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